THE EFFECTS OF PLASTICIZER TREATMENT ON INFLAMMATION AND WOUND HEALING

dc.contributor.advisorHarper, James
dc.creatorZia, Irma
dc.creator.orcid0000-0002-6875-2116
dc.date.accessioned2020-12-10T14:00:49Z
dc.date.available2020-12-10T14:00:49Z
dc.date.created2020-12
dc.date.issued2020-11-13
dc.date.submittedDecember 2020
dc.date.updated2020-12-10T14:00:49Z
dc.description.abstractPlasticizers are man-made chemicals used in the manufacture of a variety of products to ensure flexibility and longevity. While these compounds allow for the formation of more durable products, they have proven to be detrimental to the health of living organisms. Dibutyl phthalate (DBP) and bisphenol A (BPA) are two widely used plasticizers that are classified as endocrine-disrupting chemicals (EDC) due to their effects on developmental, endocrinological, reproductive, and metabolic function. We sought to investigate the effect of these compounds on the inflammatory response in vitro using a mouse macrophage cell line (RAW 264.7). Additionally, we used a live animal model (C57BL/6J mice) to determine the effects of DBP on the wound healing response. These studies indicated that high doses of either DBP or BPA altered the total cell count with time with or without exposure to bacterial lipopolysaccharide (LPS). When used in combination, an even greater effect was seen. We also found that treatment with these compounds, alone and/or in conjunction, had a significant effect on the measurable level of the cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) after LPStreatment in RAW 264.7 cells. However, DBP did not affect wound healing in a mouse model, although there was a marked increase in the circulating level of TGF-β treated with 100 mg DBP/kg body weight. Overall, these data suggest that plasticizers can influence the inflammatory response which may have implications for human health.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.11875/2905
dc.language.isoen
dc.subjectDibutyl phthalate
dc.subjectBisphenol A
dc.subjectInflammation
dc.subjectPlasticizers
dc.subjectLipopolysaccharide
dc.subjectWound healing
dc.subjectEndocrine disruptors
dc.titleTHE EFFECTS OF PLASTICIZER TREATMENT ON INFLAMMATION AND WOUND HEALING
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentBiological Sciences
thesis.degree.grantorSam Houston State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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