Effect of Cell Culture Condition or Bisphenol A (BPA) Exposure on the Wound Healing Response In Vitro
Wound healing is a process comprised of overlapping phases: inflammation, tissue formation, reepithelization, and remodeling; all of which can be influenced by exogenous factors such as environmental pollutants. Bisphenol A (BPA), a monomer used in the manufacture of many plastics, is an endocrine-disrupting chemical that may influence cell proliferation and/or cellular migration during wound repair. Here I used an in vitro model of wound healing to investigate the effect of varying the composition of the cell culture media in conjunction with BPA exposure to determine how each of these factors may influence the kinetics of the wound healing response. Specifically, the degree of closure over 24 hours was measured in the presence or absence of bovine serum in the cell culture media in cells pre-exposed to varied concentrations of BPA. Using an algorithm developed for ImageJ to autonomously estimate the rate of wound closure, we found that the presence of serum in the culture media significantly increased this rate in (p ≤ 0.001). Prior exposure to BPA had no effect on the rate of wound closure, regardless of dose or cell culture condition (p ≥ 0.05). Collectively, these data suggest that serum growth factors are essential mediators of wound closure in vitro, whereas BPA exposure has no significant effect.