Identification and antimicrobial properties of Pseudomonas from soil

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dc.contributor.advisorLynne, Aaron
dc.creatorGaffney, Caitlyn Mary
dc.date.accessioned2020-12-10T14:00:30Z
dc.date.available2020-12-10T14:00:30Z
dc.date.created2020-12
dc.date.issued2020-11-16
dc.date.submittedDecember 2020
dc.date.updated2020-12-10T14:00:31Z
dc.description.abstractAs the emergence of multi-drug resistant bacteria are continuously rising, there is a dire need for novel antibiotics. As pharmaceutical companies have become less involved in the discovery of new antibiotics, alternative resources have been explored. The Small World Initiative (SWI) has teamed up with the Centers for Disease Control & Prevention (CDC) and the National Institutes of Health (NIH) to help alleviate the growing global antibiotic crisis of multi-drug resistant bacteria. The SWI has encouraged many around the country to search for novel antibiotics in the soil bacteria. In this study we isolated antibiotic producing Pseudomonas isolates from soil in the Piney Woods. Over 300 isolates screened showed antibiotic properties against ESKAPE pathogens and of those, 30 isolates showed antibiotic properties against five clinical multi-drug resistant Salmonella strains. Additionally, we were able to extract antimicrobial compounds from the isolates and show inhibition towards five clinical multi-drug resistant Salmonella strains. Lastly, we did whole genome sequencing on ten of the antibiotic producing Pseudomonas isolates and compared the genomes of three to identify homologous genomic features for antibiotic production. Through genomic comparisons we were able to find unique features in the genomes that could potentially explain the varying inhibition abilities amongst the three isolates.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.11875/2904
dc.language.isoen
dc.subjectPseudomonas
dc.subjectMulti-drug resistance
dc.subjectESKAPE pathogens
dc.subjectAntibiotics
dc.subjectDrug discovery
dc.subjectWhole-genome sequencing
dc.subjectComparative genomics
dc.titleIdentification and antimicrobial properties of Pseudomonas from soil
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentBiological Sciences
thesis.degree.grantorSam Houston State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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